Non-viral gene transfer into murine adipose derived mesenchmal stem cells: a comparative study of different non-viral methods.

چکیده مقاله

Background: Bone is a tissue with high repair capacity; but, this ability dramatically is limited during irrecoverable bone and skeletal defects. Stem cell therapy is a promising approach for construction of bone tissue. Mesenchymal stem cells (MSCs) have been introduced as basic tools for bone tissue generation in tissue engineering. Core binding factor alpha 1 (Cbfa1) is one of the main genes involved in osteogenesis. Since relative expression of Cbfa1 in differentiated osteoblasts from adipose derived stem cells is unknown, this study aimed to evaluate the gene expression pattern of Cbfa1 in adipose derived differentiated osteoblasts in 4 different time intervals. Methods: Real-time PCR was used for studying the gene expression of Cbfa1 in human normal osteoblasts and adipose derived osteogenic osteoblasts at the days 7, 14, 21 and 28. Findings: There was a progressive increase in Cbfa1 expression over the differentiation period of adipose derived mesenchymal stem cells (ADSCs) from day 7 to day 28. Differentiated osteoblasts expressed Cbfa1 lower than native osteoblasts (P < 0.05). Conclusion: ADSCs are an attractive source for engineering a new bone tissue. According to our results, adipose derived differentiated osteoblasts express candidate gene at least on day 7 of culture. Our study indicates that longer treatment of ADSCs in osteogenic medium confer better osteoblastic properties to these cells.