Cell Culture and Cryopreservation of Sexual Tissues of Three Iranian leopard Cheetahs

چکیده مقاله

Background: The conservation of the wildlife animals, as the valuable genetic reserves, is one of the important policies of the countries. This issue becomes more important especially in relation to endangered species. The Iranian cheetah is one of the species that is seriously endangered. In this study, in order to preserve the genetic and biological resources of the country, we extracted the cells from the skin and testicle tissue, as well as cryopreservation the ovary and testicle tissue of 3 Iranian leopard Cheetahs. Materials and Methods: Immediately after the death of Iranian cheetah, skin tissue and sexual tissue samples were taken from the animal. The samples were transferred to the laboratory in the shortest time in the culture medium containing antibiotics at 4°C. In the laboratory, skin tissue and some testicular tissues were cultured by explant culture and enzymatic digestion, and some testicular and ovarian tissues were cryopreserved by slow method. Results: The cells extracted from skin tissue and testicle tissue were frozen after 4 weeks of culture after quality control tests. The frozen ovary and testis tissues were stored in a nitrogen tank for long-term storage. Conclusion: Cryopreservation the cells and sex tissue of endangered animals such as the Iranian leopard cheetah is a way to preserve their genomic information and hope for the revival of this endangered species. Keywords: Conservation, Cryopreservation, Endangered Species, Sexual Ti