| نویسندگان | Zahra Elyasi Gorji, Kourosh J. Khaledi, Abdolreza Daneshvar Amoli, Meysam Ganjibakhsh, Ahmad Nasimian, Neda Sadat Gohari, Mehrnaz Izadpanah, Faezeh Vakhshiteh, Maryam Farghadan, Shiva Mohammadi Moghanjoghi, Hedieh Rahmati, Abolhassan Shahzadeh Fazeli, Par |
|---|---|
| نشریه | Conservation genetics resources |
| نوع مقاله | Full Paper |
| تاریخ انتشار | 2017 |
| رتبه نشریه | ISI |
| نوع نشریه | چاپی |
| کشور محل چاپ | آلمان |
چکیده مقاله
Cryopreservation of cell lines from endangered animals is an important approach to conserve genetic resources. In this study, ear ibroblasts from 51 Iranian Sistani cattle (Bos indicus) were cultured and cryopreserved in vitro, and their biological characteristics, proliferation and chromosomal features were analyzed. The results indicated that Sistani cattle ibroblasts could be successfully obtained by explant culture. The cultured cells were typical ibroblasts, with normal morphology and population doubling time of approximately 24 ± 0.5 h. Karyotyping showed that frequency of cells with chromosome number 2n = 60 was >95%. Bacterial, fungal, yeast and mycoplasma contaminations were negative. The eiciencies of VSV-G (pMDG) and lentiviral pCSGW vectors encoding luorescent proteins were around 60%. Multiplex PCR for identiication of species conirmed that Sistani cattle cell lines had no cross-contamination. The samples identiication information was prepared and is available in this research. These established cell lines are valuable genetic pools from endangered Iranian Sistani cattle breed, and would be a source of many biological studies such as genomic, postgenomic and somatic cell cloning researches