Enhancement of the rates of CTCs trapping by integrating nanowire substrate and EpCAM antibody conjugated with magnetite nanoparticles in a microfluidic device

چکیده مقاله

The detachment of some tumor cells from a tissue and their penetration into the bloodstream at very low concentrations led to the phenomenon of circulating tumor cells (CTC). One of the reliable and valid methods is to separate and isolate CTCs by magnetophoresis method in microfluidic channel. In recent years, the use of nanostructured platforms to trap and isolate tumor cells targeted with specific markers has been considered. Here, our purpose is expanding a pioneer technique to trap and isolate MCF-7 cell lines as an experimental model of CTCs in the blood that integrates the magnetophoretic property of some paramagnetic nanoparticles with a silicon nanowires substrate in a microfluidic device. The simulation represents that the scheme of the device can trap approximately entire of CTCs on the nanowires substrate, while trapping with a smooth substrate and with the magnetophoretic force does not performed completely and comprehensively. Experimental validation of specific binding to tumor cells was performed by anit-EpCAM antibody. Trapping efficiency above 93% using this integration scheme and with specific binding of antibodies to nanoparticles is one of the most prominent results of this design.